α-Naphthyl Butyrate Esterase (α-NBE) Stain

Release time:2016-8-8      Reads:3589

α-Naphthyl Butyrate Esterase (α-NBE) Stain

Intended Use:

This kit is for staining bone marrow cell and bloodcell smear.

 

Principle:

Under alkaline condition,  α-Naphthyl Butyrate is hydrolyzed into  α-naphthol by Esterase.  α-naphthol shall combine with diazonium salt to forminsoluble colored precipitate in cytoplasm. This stain has no specificity against esterase; therefore, it is called"Non-specific Esterase Stain".

 

Specifications:

 

Contents

5Tests/Kit

20Tests/Kit

100Tests/Kit

Components

Fixative
(solution A)

1vial x 5ml

1vial x 20ml

1vial x 100ml

Sodium Citrate

Pararosaniline
(solutionB)

1vial x 0.1ml

1vial x 0.3ml

1vial x 1.1ml

Parafuchsin

Sodium Nitrite
(solution C)

1vial x 0.1ml

1vial x 0.3ml

1vial x 1.1ml

Sodium Nitrite

Phosphate Buffer
(solution D)

1vial x 25ml

1vial x 90ml

2vials x 200ml

Phosphate

α-Naphthyl Butyrate
(solution E)

1vial x 1ml

1vial x 4.5ml

1vial x 21ml

α-Naphthyl
Butyrate

Methyl Green
(solution F)

1vial x 5ml

1vial x 20ml

1vial x 100ml

Methyl Green

 

Methods:

Working solution Preparation (for 1 test only):

Apparatus required: disposable tube, micropipette, disposable tips, dropper;

Instruction: Add 10µl solution B to 10µl solution C, mix well and wait for 1 minute;

Add 4ml solution D and 200µl solution E. Mix well (Add 2 drops of solution NaFfor NaF inhibition test.)

 

 

Solution B

Solution C

Solution D

Solution E

Solution
NαF

Tube  Α

10µl

10µl

4ml

200µl

-

Tube B
(inhibition test)

10µl

10µl

4ml

200µl

2drops

 

Expected Results:

Red or red-brown granules found in cytoplasm means positive.

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