AS-DCE Stain

Release time:2016-8-8      Reads:2538

Naphthol AS-D Chloroacetate Esterase (AS–DCE) Stain

Intended Use:

This kit is for staining bone marrow cell and bloodcell smear.

 

Principle:

Naphthol AS-D chloroacetate can be hydrolyzed into  Naphthol AS-D by Esterase to mix with diazonium salt and form insoluble red-brown precipitate in cytoplasm. Positive reaction is only found in granulocytes; hence, it is also called "Specific Esterase Stain".

 

Specifications:

 

Contents 5Tests/Kit 20Tests/Kit 100Tests/Kit Components
Fixative
(solution A)
1vial×2.5m 1vial×10ml 1vial×50ml Sodium Citrate
Pararosaniline
(solution B)
1vial×0.1ml 1vial×0.3ml 1vial×1.1ml Parafuchsin
Sodium Nitrite
(solution C)
1vial×0.1ml 1vial×0.3ml 1vial×1.1ml Sodium Nitrite
Phosphate Buffer
(solution D)
1vial×5ml 1vial×20ml 1vial×95ml Phosphate
Naphthol As-D
Chloroacetate
(solution E)
1vial×0.3ml 1vial×1.2ml 1vial×5.5ml Naphthol
AS-D
hloroacetate
Methyl Green
(solution F)
1vial×5ml 1vial×20ml 1vial×100ml Methyl Green

 

Methods:

Working solution Preparation(for 1 test only);

Apparatus required: disposable tube, micropipette, disposable tips, dropper;

Instruction: Mix 10µl solution B and10µl solution C thoroughly. Wait for 1 Minute;

Add 0.9ml solution D and 50µl solution E. Mix well;

 

Expected Results:

Red granules found in cytoplasm means positive.

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