Neutrophilic- Leukocyte Alkaline Phosphatase Stain

Release time:2016-8-10      Reads:6679

Neutrophilic- Leukocyte Alkaline Phosphatase (NAP) Stain

 

Intended Use:

This kit is for staining bone marrow cell and bloodcell smear.

 

Principle:

Neutrophilic-Leukocyte Alkaline Phosphatase stain is based on diazotization-coupling principle. At pH9.2~9.8, Alkaline Phosphatase hydrolyzes Naphthol AS-BI Phosphoric Acid into  α-naphthol. The stable diazonium salt and naphthol shall form insoluble colored precipitate in cytoplasm.

 

Specifications:

Contents 5Tests/Kit 20Tests/Kit 100Tests/Kit Components
Fixative (solution A) 1vial×5ml 1vial×20ml 1vial×100ml Sodium Citrate
FBB -Alkaline
(solution B)
1vial×0.3ml 1vial×1.1ml 1vial×5.1ml Fast Blue BB
Base
Sodium Nitrite
(solution C)
1vial×0.3ml 1vial×1.1ml 1vial×5.1ml Sodium Nitrite
Naphthol AS-Bl Alkaline
(solution D)
1vial×0.3ml 1vial×1.1ml 1vial×5.1ml Naphthol AS-Bl
 Neutral Red 
(solution E)
1vial×5ml 1vial×20ml 1vial×100ml Neutral Red

 

Methods:

Working solution Preparation (for 1 test only)

Apparatus required:disposable tube, micropipette, disposable tips, dropper;

Instruction:Mix 25µl solution B with 25µl C; wait for 2 minutes. Add distilled water 2ml into 25µl solution D. Mix thoroughly.

 

Expected Results:

NΑP positive granules are blue.

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